CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: MEM-25]

CAT#: BM4046

CD11a (ITGAL) mouse monoclonal antibody, clone MEM-25, Purified

Specifications

Product Data

• Clone Name: MEM-25
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Immunohistochemistry on frozen sections (0.2 ug/ml, 1/2000).
  Flow Cytometry.
  Immunoprecipitation.
  Suggested positive control: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Mixed leukocyte preparation.
• Specificity: MEM-25 recognizes the CD11a subunit of the αLβ2 integrin heterodimer, also called lymphocyte-function-associated antigen 1 (LFA-1).
  CD11a is a general marker of leucocytes in immune and other tissues.
  Does not react with non-hemopoietic tissue and platelets.
  The antibody blocks binding of CFA-1 to ICAM-1.
• Formulation: PBS buffer pH 7.2 with 0.05% sodium azide as preservative and 10 mg/ml BSA as stabilizer.
  State: Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with 0.6 ml distilled water.
• Purification: Affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Do not freeze working dilutions
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: integrin subunit alpha L
• Database Link:
  Entrez Gene 3683 Human
  UniProt ID P20701
• Background: CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.
• Synonyms: Integrin alpha-L, LFA1, LFA-1
• Note: Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer's hemalum.