CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: MEM-25]

CAT#: BM4046

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CD11a (ITGAL) mouse monoclonal antibody, clone MEM-25, Purified



USD 501.00


Availability*
2 Weeks

Size
    • 200 ug


Product images

Specifications

Product Data
Clone Name MEM-25
Applications FC, FN, IHC, IP
Recommended Dilution Immunohistochemistry on frozen sections (0.2 ug/ml, 1/2000).
Flow Cytometry.
Immunoprecipitation.
Suggested positive control: Human tonsil.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Mixed leukocyte preparation.
Specificity MEM-25 recognizes the CD11a subunit of the αLβ2 integrin heterodimer, also called lymphocyte-function-associated antigen 1 (LFA-1).
CD11a is a general marker of leucocytes in immune and other tissues.
Does not react with non-hemopoietic tissue and platelets.
The antibody blocks binding of CFA-1 to ICAM-1.
Formulation PBS buffer pH 7.2 with 0.05% sodium azide as preservative and 10 mg/ml BSA as stabilizer.
State: Purified
State: Lyophilized purified IgG fraction.
Reconstitution Method Restore with 0.6 ml distilled water.
Purification Affinity chromatography.
Conjugation Unconjugated
Storage Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Gene Name integrin subunit alpha L
Background CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.
Synonyms Integrin alpha-L, LFA1, LFA-1
Note Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum.
Reference Data
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