CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: MEM-25]
CD11a (ITGAL) mouse monoclonal antibody, clone MEM-25, Purified
|Applications||FC, FN, IHC, IP|
|Recommended Dilution||Immunohistochemistry on frozen sections (0.2 ug/ml, 1/2000).
Suggested positive control: Human tonsil.
|Immunogen||Mixed leukocyte preparation.|
|Specificity||MEM-25 recognizes the CD11a subunit of the αLβ2 integrin heterodimer, also called lymphocyte-function-associated antigen 1 (LFA-1).
CD11a is a general marker of leucocytes in immune and other tissues.
Does not react with non-hemopoietic tissue and platelets.
The antibody blocks binding of CFA-1 to ICAM-1.
|Formulation||PBS buffer pH 7.2 with 0.05% sodium azide as preservative and 10 mg/ml BSA as stabilizer.
State: Lyophilized purified IgG fraction.
|Reconstitution Method||Restore with 0.6 ml distilled water.|
|Storage||Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions
Avoid repeated freezing and thawing.
|Stability||Shelf life: One year from despatch.|
|Gene Name||integrin subunit alpha L|
|Background||CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.|
|Synonyms||Integrin alpha-L, LFA1, LFA-1|
|Note||Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.