Cd68 Mouse Monoclonal Antibody [Clone ID: ED1]

CAT#: BM4000S

Cd68 mouse monoclonal antibody, clone ED1, Purified

Product Images

Figure 2. Staining of ED1 antibody on Rat spleen (frozen section)

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  Figure 1. Staining of rat liver, with induced hepatocellular damage, with Mouse anti Rat CD68 antibody

Specifications

Product Data

• Clone Name: ED1
• Applications: FC, IF, IHC, IP, R, WB
• Recommended Dilution: RIA.
  Western blotting.
  Immunoprecipitation.
  Immunofluorescence.
  Flow Cytometry: Use 10 µl of 1/25-1/100 diluted CD68 antibody to label 10⁶ cells in 100 µl (Membrane permeabilisation is required for best results).
  Immunohistology on Frozen Sections.
  Immunohistology on Paraffin Sections: 1/50-1/100. Requires protein digestion pretreatment of Paraffin sections with Pronase or Trypsin.
• Reactivities: Bovine, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat spleen cells.
  Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
• Specificity: CD68 Antibody (Cat#BM4000) recognises a single chain glycoprotein of 110 kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life one year from despatch.
• Gene Name: Cd68 molecule
• Database Link:
  Entrez Gene 287435 Rat
  UniProt ID Q4FZY1
• Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
• Synonyms: Gp110, Macrosialin, Macrophage marker
• Note: Studies have shown that the antigen recognised by ED1 is the Rat homologue of Human CD68.