Cd68 Mouse Monoclonal Antibody [Clone ID: ED1]

CAT#: BM4000B

Cd68 mouse monoclonal antibody, clone ED1, Biotin

Specifications

Product Data

• Clone Name: ED1
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
  (Results maybe enhanced using membrane permeabilisation).
  Immunohistochemistry on Frozen sections: 1/10.
• Reactivities: Bovine, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat spleen cells
• Specificity: CD68 antibody (Cat.-No BM4000B) recognizes a single chain glycoprotein of 110kDa that is expressed predominantly on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages, and weakly be peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of Human CD68.
  Negative Species: Horse.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Cd68 molecule
• Database Link:
  Entrez Gene 287435 Rat
  UniProt ID Q4FZY1
• Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
• Synonyms: Gp110, Macrosialin, Macrophage marker