Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: Ks18.04]

CAT#: BM2275P

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone Ks18.04, Purified

USD 441.00

2 Weeks*

    • 50 ug

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Product Data
Clone Name Ks18.04
Applications IHC, WB
Recommended Dilution Western blot.
Immunohistochemistry on Frozen and Paraffin Sections: 1/20 with PBS. 
Microwave treatment is recommended.
Incubation time: 1h at 37°C, extended with paraffin sections (overnight at 2-8°C).
Reactivities Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout, Zebrafish
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human keratin K18 from HeLa cytoskeletal preparation
Specificity Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.
Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Tested Reactivities on Cultured Cell Lines: HeLa and MCF-7.
Formulation PBS, pH 7.4
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 0.5% BSA
Preservative: 0.09% Sodium azide
Reconstitution Method Restore in 1.0 ml distilled water.
Purification Affinity Chromatography on Protein A
Conjugation Unconjugated
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Synonyms Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data

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