Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: Ks18.04]

CAT#: BM2275F

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone Ks18.04, FITC

Product Images

BM2275F KRT18 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Colon followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  BM2275F KRT18 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Placenta followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  BM2275F KRT18 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Prostate followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: Ks18.04
• Applications: FC, IF, IHC
• Recommended Dilution: Immunofluorescence.
  Cytological material.
  Flow Cytometry: 1/5-1/10.
  Immunohistochemistry on Frozen Sections: 1/10.
  Immunohistochemistry on Paraffin Sections: 1/10.
  Incubation Time: 1 h at 37°C.
• Reactivities: Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human keratin K18 from a HeLa cytoskeletal preparation
• Specificity: Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.
  Tested Tumors Specifically Detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
  Polypeptide Reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
  Reacts with HeLa and MCF-7 (cell lines tested so far).
• Formulation: Label: FITC
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein
• Purification: Affinity Chromatography on Protein A
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3875 Human
  UniProt ID P05783
• Background: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
• Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18