Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: Ks18.04]

CAT#: BM2275F

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Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone Ks18.04, FITC



USD 487.00


Availability*
2 Weeks

Size
    • 250 ul


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Specifications

Product Data
Clone Name Ks18.04
Applications FC, IF, IHC
Recommended Dilution Immunofluorescence.
Cytological material.
Flow Cytometry: 1/5-1/10.
Immunohistochemistry on Frozen Sections: 1/10.
Immunohistochemistry on Paraffin Sections: 1/10.
Incubation Time: 1 h at 37°C.
Reactivities Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human keratin K18 from a HeLa cytoskeletal preparation
Specificity Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.
Tested Tumors Specifically Detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
Polypeptide Reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Reacts with HeLa and MCF-7 (cell lines tested so far).
Formulation Label: FITC
State: Liquid purified Ig fraction
Preservative: 0.09% Sodium Azide
Label: Fluorescein
Purification Affinity Chromatography on Protein A
Conjugation FITC
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Synonyms Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data
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