Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: Ks18.04]
Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone Ks18.04, FITC
|Applications||FC, IF, IHC|
Flow Cytometry: 1/5-1/10.
Immunohistochemistry on Frozen Sections: 1/10.
Immunohistochemistry on Paraffin Sections: 1/10.
Incubation Time: 1 h at 37°C.
|Reactivities||Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout|
|Immunogen||Human keratin K18 from a HeLa cytoskeletal preparation|
|Specificity||Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.
Tested Tumors Specifically Detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.
Polypeptide Reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.
Reacts with HeLa and MCF-7 (cell lines tested so far).
State: Liquid purified Ig fraction
Preservative: 0.09% Sodium Azide
|Purification||Affinity Chromatography on Protein A|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.|
|Synonyms||Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.