Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody [Clone ID: KS20.10]

CAT#: BM2247

Cytokeratin 20 (KRT20) mouse monoclonal antibody, clone KS20.10, Purified

Product Images

Keratin 20: Overview

Specifications

Product Data

• Clone Name: KS20.10
• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on paraffin Embedded Sections (Only after after microwave treatment). Dilute 1/10 with PBS for immunohistochemistry.
  Incubation time: 1h at RT, orover night at 4°C.
  Western blotting.
  ELISA.
• Reactivities: Human, Porcine, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Electrophoretically purified Keratin K20 from Human intestinal mucosa.
• Specificity: IT-Ks 20.10 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin.
  Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma).
  Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
  Polypeptide recognized: protein IT (keratin K20; Mr 46 000; formerly also designated cytokeratin 20).
  Tested Reactivities on Cultured Cell Lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
  Negative Species: Mouse.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1 ml distilled water.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 20
• Database Link:
  Entrez Gene 54474 Human
  UniProt ID P35900
• Background: Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.
• Synonyms: Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT