Niemann Pick C1 (NPC1) Goat Polyclonal Antibody

CAT#: AP60000PU-N

Niemann Pick C1 (NPC1) goat polyclonal antibody, Aff - Purified

Product Images

(0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: 0.5-1.5 μg/ml. Approx 170kDa band observed in lysates of cell line HEK293 (calculated MW of 142kDa according to NP_000262.2).
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-TYHTVLQTSADFID, from the internal region of the protein sequence according to NP_000262.2.
• Specificity: This antibody recognizes Human NPC1.
  Other species not tested.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 4864 Human
  UniProt ID O15118
• Synonyms: FLJ98532; NPC; NPCA1