Delta-2 Tubulin (Detyrosinated & Decarboxylated alpha-Tubulin) Rabbit Polyclonal Antibody

CAT#: AP54886SU-N

Delta-2 Tubulin (Detyrosinated & Decarboxylated alpha-Tubulin) rabbit polyclonal antibody, Serum

Product Images

AP54886SU-N Delta-2 Tubulin Antibody Staining of Human neurone

• 

  AP54886SU-N Delta-2 Tubulin Antibody Staining of Human neurone

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution:

  ELISA.
  Western Blot: 1/500-1/2000.
  Immunohistochemistry: 1/50-1/200.

• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptides derived from C-terminal domain of Human Tubulin
• Specificity: Reacts with Delta-2 Tubulin (Detyrosinated and Decarboxylated alpha-Tubulin).
• Formulation: State: Serum
  State: Lyophilized powder
  Preservative: None
• Reconstitution Method: Restore in distilled water.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").