RNF103-CHMP3 Rabbit Polyclonal Antibody

CAT#: AP54843PU-N

RNF103-CHMP3 rabbit polyclonal antibody, Purified

Product Images

Prostate stained with VPS24 antibody Cat.-No AP54843PU-N in Immunohistochemistry on Paraffin Sections.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Immunohistochemistry.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from internal domain of Human Vps24 protein
• Specificity: Reacts with 25 kDa hVps24.
• Formulation: 0.1M Tris, 0.1M Glycine and 2% Sucrose
  State: Purified
  State: Lyophilized purified antibody
  Preservative: None
• Reconstitution Method: Restore in distilled water.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store the antibody at -20°C.
  Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 100526767 Human
  UniProt ID Q9Y3E7
• Background:

  The charged multivesicular body proteins, commonly designated CHMPs, belong to the vacuolar sorting protein family and function as chromatin-modifying proteins. CHMP1-6 are all components of ESCRT (endosomal sorting complex required for transport) I, II or III complexes. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs), as well as required for the formation of these bodies. CHMP3, also known as Vps24, associates directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. Overexpression of CHMP3 strongly inhibits HIV-1 release.
  CHMP3 is expressed in brain, skeletal muscle, heart, lung, kidney and liver.

• Synonyms: CHMP3, NEDF, CGI-149