HBA1 (Center) Rabbit Polyclonal Antibody

CAT#: AP52006PU-N

HBA1 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of HBA2 Antibody (Center) in mouse liver tissue lysates (35ug/lane). HBA2 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HBA2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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  Flow Cytometric analysis of K562 cells using HBA2 Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1000.
  Western Blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 100~128 amino acids from the Central region of human Hemoglobin alpha
• Specificity: This antibody recognizes Human and Mouse Hemoglobin alpha (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: hemoglobin subunit alpha 1
• Database Link:
  Entrez Gene 3040 HumanEntrez Gene 3039 Human
  UniProt ID P69905
• Background: HBA2 located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
• Synonyms: HBA1, Alpha-globin, Hemoglobin alpha chain
• Note: Molecular Weight: 15258 Da