beta glucuronidase (GUSB) (Center) Rabbit Polyclonal Antibody

CAT#: AP51986PU-N

beta glucuronidase (GUSB) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Beta-glucuronidase Antibody (Center) in WiDr, A2058, K562 cell line lysates (35ug/lane).

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  Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human colon carcinoma reacted with Beta-glucuronidase Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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  Flow cytometric analysis of WiDr cells using Beta-glucuronidase Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Western Blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Porcine
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 335 - 362 amino acids from the Center region of Human Beta-glucuronidase
• Specificity: This antibody recognizes Human Beta-glucuronidase (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: glucuronidase beta
• Database Link:
  Entrez Gene 2990 Human
  UniProt ID P08236
• Background: The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).
• Synonyms: GUSB, Beta-G1
• Note: Molecular Weight: 74732 Da

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - other enzymes, Glycosaminoglycan degradation, Lysosome, Metabolic pathways, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Starch and sucrose metabolism