beta Catenin (CTNNB1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP51124PU-N

beta Catenin (CTNNB1) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of CTNB1 (arrow) using Catenin beta-1 Antibody (C-term). 293 cell lysates (2 ug/lane) either non-transfected (Lane 1) or transiently transfected (Lane 2) with the CTNB1 gene.

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  Formalin fixed, paraffin embedded human stomach tissuem stained with Catenin beta-1 Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CTNB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of Catenin beta-1 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 653-681 amino acids from the C-terminal region of Human Catenin beta-1.
• Specificity: This antibody recognizes Human Catenin beta-1 (C-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Gene Name: catenin beta 1
• Database Link:
  Entrez Gene 1499 Human
  UniProt ID P35222
• Background:
  The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.
• Synonyms: CTNNB1, CTNNB, Beta-catenin
• Note: Molecular Weight: 85497 Da

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway