GST-Tag Rabbit Polyclonal Antibody

CAT#: AP33449PU-N

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GST-Tag rabbit polyclonal antibody, Aff - Purified



USD 242.00


Availability*
2 Weeks

Size
    • 50 ug


Product images

Specifications

Product Data
Applications WB
Recommended Dilution Western blot: 1-5 µg/ml.
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Recombinant GST-tag protein.
The anti-GST-tag antibody was generated by immunization of rabbits with a highly purified GST-tagged protein produced in E. coli.
Specificity GST-tag.
Formulation PBS, pH 7.4, without preservatives
State: Aff - Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Antigen Affinity Chromatography
Conjugation Unconjugated
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Synonyms GST26-Tag, Glutathione S-transferase Tag
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.