GFP Chicken Polyclonal Antibody

CAT#: AP31791PU-N

GFP chicken polyclonal antibody, Aff - Purified

Product Images

The same murine cortical neuron visualized using our Chicken anti-GFP antibody (left, in red) and visualized using GFP autofluorescence (right, in green). Note the increased detail and intensity of the anti-GFP antibody staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot (1/2500-1/5000).
  Immuofluorescence.
  Immunohistochemistry (1/500-1/1000).
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Purified recombinant Green Fluorescent Protein (GFP) emulsified in Freund's adjuvant.
  After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks.
  Purified antibody preparations were then mixed with Glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with Thimerosal, and then filter-sterilized.
• Specificity: Recognizes Green Fluorescent Protein (GFP).
  Quality Control: Antibodies were analyzed by Western blot analysis (1/5000) and Immunohistochemistry (1/500) using transgenic mice expressing the GFP gene product.
  Western blots were performed using BlokHen® as the blocking reagent, and HRP-labeled Goat anti-Chicken antibody (Cat.-No AP31795HR-N) as the detection reagent.
  Immunohistochemistry used Tetramethyl Rhodamine-Labeled anti-Chicken IgY.
• Formulation: Sodium Phosphate-Buffered (10 mM, pH7.2) isotonic (0.9%, w/v) Saline with 50% (v/v) Glycerol as an anti-freezing agent and 0.02% (w/v) sodium azide as an anti-microbial agent.
  State: Aff - Purified
  State: Liquid purified IgY fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C in the dark.
  Avoid repeated freezing and thawing.
  Centrifuge vial before opening.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P42212
• Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
• Synonyms: Green fluorescent protein, GFP-Tag