Complement C3 (C3) Rabbit Polyclonal Antibody

CAT#: AP31520SU-N

Complement C3 (C3) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3b or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
  Directions for use:
  In immunoelectrophoresis use 2 μl human plasma or equivalent against 120 μl antiserum.
  In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  In single radial immunodiffusion use 1 percent antiserum in the gel.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: C3b (C3c+C3d) has a molecular weight of 170,000. The protein is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.
• Formulation: No preservative added. - No foreign proteins added.
  State: Serum
  State: Delipidated, heat inactivated, lyophilized, stable whole serum.
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: complement component 3
• Database Link:
  Entrez Gene 717 HumanEntrez Gene 718 Human
  UniProt ID P01024
• Synonyms: CPAMD1, complement component 3
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus