Albumin Rabbit Polyclonal Antibody

CAT#: AP31513SU-N

Albumin rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used as precipitating antiserum to identify or measure monkey albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell).
  It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
  Recommended dilutions:
  Immunoelectrophoresis: use 2 μl serum, plasma or equivalent against 120 μl antiserum.
  Double radial immunodiffusion (Ouchterlony): use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  Single radial immunodiffusion and electroimmunodiffusion: use 0.5 to 1.0 percent antiserum in the agar gel.
• Reactivities: Monkey
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified albumin isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The defined antibody specificity is directed to albumin as tested against monkey sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against monkey serum and the purified albumin.
• Formulation: Without preservatives and foreign proteins.
  State: Serum
  State: Delipidated, heat inactivated, lyophilized, stable whole antiserum
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID A2V9Z4
• Background: Serum albumin, a globular unglycosylated protein of 65 kDa, is the main protein of plasma; it has a good binding capacity for water, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to give the secreted albumin.
• Synonyms: ALB, BSA, HSA, Serum Albumin
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
  Antibody titre: Precipitin titre not less than 1/32 when tested against normal monkey serum in agar block immunodiffusion titration.