Tnni3 pSer23/24 Rabbit Polyclonal Antibody

CAT#: AP26442PU-N

Tnni3 pSer23/24 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot of mouse heart homogenate showing sp ecific immunolabeling of the ~25k cTnI protein phosphorylated at Ser 23/24 (control). Immunolabeling is blocked by preadsorption with the phos pho-peptide used as antigen (Peptide), but not by the corresponding dephospho-peptide (not shown).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/1000.
• Reactivities: Human, Mouse, Primate, Rat
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser 23/24 of mouse troponin I, cardiac (cTnI)
• Specificity: Specific for the ~25k cardiac troponin I protein phosphorylated at Ser 23/24.
• Formulation: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Purification: Affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: troponin I, cardiac 3
• Database Link:
  Entrez Gene 21954 Mouse
  UniProt ID P48787
• Background: Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place, and wh en cTnI is phosphorylated by protein kinase C and protein kinase A at Ser 23/24 it causes regulation of Ca 2+ - stimulated ATPase (Noland et al, 1995). Evidence suggests that phosphorylation of both se rines 23 and 24 is required for the reduction in Ca 2+ - sensitivity and beneficial for relaxation of the heart (Kooij et al, 2010). Ser 23/24 phosphorylation is important for enhanced relaxation in response to prol onged activation of protein kinase C by endothelin in intact myocytes, while Thr 144 plays an important role in the acute acceleration of relaxation (Westfall et al, 2005).
• Synonyms: TNNI3, TNNC1