Tnni3 Rabbit Polyclonal Antibody

CAT#: AP26440SU-N

Tnni3 rabbit polyclonal antibody, Serum

Product Images

Western blot of 20 ug of mouse heart lysate showing specific immunolabeling of the ~25k cardiac troponin I protein.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/2000 (although higher dilutions can sometimes be used as cardiac troponin I expression is quite high in cardiac muscle).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Fusion protein of the mouse cardiac troponin I holoprotein
• Specificity: Specific for the ~25k cardiac troponin I protein.
• Formulation: State: Serum
  State: Liquid unpurified neat serum
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: troponin I, cardiac 3
• Database Link:
  Entrez Gene 21954 Mouse
  UniProt ID P48787
• Background: Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the ca lcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by ext ension enhances rates of cross-bridge cycling and diastolic relaxation (Noland, Jr. et al., 1995; Noland et al., 1989). In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosp horylation of TnI (largely at Ser43) inhibits the actin-cross bridge reaction and reduces the Ca ++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation (Noland, Jr. and Kuo, 1991). Phosphorylation at Thr144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates (Sumandea et al., 2008). Importantly, changes in th e phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression (Walker et al., 2010).
• Synonyms: TNNI3, TNNC1