VEGFA (N-term) Rabbit Polyclonal Antibody

CAT#: AP23353PU-N

VEGFA (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat cardiac muscle tissue lysis using VEGF antibody

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  Immunohistochemical analysis of paraffin embedded ESCC sections, staining VEGF in cytoplams, DAB chromogenic reaction

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: Use at 0.1-0.5 μg/ml with the appropriate system to detect VEGF in cells and tissues.
  Immunohistochemistry on Paraffin Sections: Use at 0.5-1 μg/ml to detect VEGF in formalin fixed and paraffin embedded tissues. Boiling the sections in 10mM citrate buffer, pH6.0, for 20mins is required.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence near the N-teminal end of VEGFA (aa 33-48 of Human origin.
• Specificity: This antibody detects VEGF-A near N-term. No cross reactivity with other proteins.
• Formulation: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na₂HPO₄
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
  Preservative: 0.05 mg Thimerosal, 0.05 mg Sodium Azide
• Reconstitution Method: Restore with 0.2 ml of distilled water to a concentration of 0.5 mg/ml.
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1
• Database Link:
  Entrez Gene 22339 MouseEntrez Gene 83785 RatEntrez Gene 7422 Human
  UniProt ID P15692
• Background: VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo.Vascular endothelial growth factor is a mitogen primarily for vascular endothelial cells. It is, however,structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. Vascular endothelial growth factor (VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo.
• Synonyms: VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway