ERK1 / ERK2 (N-term) Rabbit Polyclonal Antibody

SKU AP23276PU-N

ERK1 / ERK2 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Data

• Application: IHC, IP, WB
• Recommended Dilution: Western blot: At 0.5-1μg/ml with the appropriate system to detect MAPK1/3 in cells and tissues.; Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect MAPK1/3 in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide mapping at the N-terminal of the human MAPK1+3
• Specificity: This antibody detects ERK1 / ERK2 at N-term. No cross reactivity with other proteins.
• Buffer: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3; State: Aff - Purified; State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Background: MAPK1(ERK2) shares high homology with MAPK3(ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein (MAP) kinases [also known as Erks] have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2.
• Synonyms: ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK

Reference Data