GAPDH (9-323) Rabbit Polyclonal Antibody

CAT#: AP23085PU-N

GAPDH (9-323) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot: Sample (10 ug of whole cell lysate). A: Adult zebrafish. 10% SDS PAGE. GAPDH antibody diluted at 1:1000.

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  Western blot: Sample (30 ug of whole cell lysate). A: 293T. B: A431. C: H1299. D: Hela. 10% SDS PAGE. GAPDH antibody diluted at 1:5000.

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  Western blot: Sample (50 ug of whole cell lysate) A: Mouse Brain. 10% SDS PAGE. diluted at 1:10000

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  Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of paraformaldehyde-fixed A549, using GAPDH antibody at 1:200 dilution.

• Immunofluorescence analysis of paraformaldehyde-fixed A549, merged with DNA probe, using GAPDH antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry: 1/100 - 1/200.
  Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/5000 - 1/20000.
• Reactivities: Bovine, Canine, Drosophila, Feline, Human, Mouse, Rabbit, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment corresponding to a region within amino acids 9 and 323 of GAPDH
• Specificity: This antibody detects GAPDH at aa 9-323.
• Formulation: PBS, 20% glycerol (pH 7), 0.025% proclin
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store (in aliquots) at -20 °C. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human
  UniProt ID P04406
• Background: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
• Synonyms: GAPD, CDABP0047
• Note: The antibody is predicted to cross react with these species based on the following homology: Rat (94%), Rabbit (94%), cow (94%), Dog (95%), Cat (96%).

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways