Creatine Phosphokinase Sheep Polyclonal Antibody
CAT#: AP21245BT-N
Creatine Phosphokinase sheep polyclonal antibody, Biotin
Conjugation: Unconjugated
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Specifications
Product Data | |
Applications | ELISA, ID, IF, IP, R, WB |
Recommended Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques). Working dilutions in non-precipitating antibody-binding techniques: 1/100-1/500. |
Reactivities | Rabbit |
Host | Sheep |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Creatine phosphokinase isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Specificity | The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
Formulation | PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized IgG fraction Molar radio: Biotin/IgG ~6.3 |
Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
Conjugation | Biotin |
Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
Stability | Shelf life: one year from despatch |
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