POLR2E Rabbit Polyclonal Antibody

CAT#: AP21061PU-N

POLR2E rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of POLR2E antibody (Cat.-No.: AP21061PU-N) in extracts from MCF-7 cells.

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  Immunohistochemistry (IHC) analyzes of POLR2E antibody (Cat.-No.: AP21061PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.
  Immunohistochemistry: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of POLR2E protein.
  (region surrounding Leu58)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 25 kDa
• Gene Name: Homo sapiens RNA polymerase II subunit E (POLR2E), transcript variant 1
• Database Link:
  Entrez Gene 66420 MouseEntrez Gene 690966 RatEntrez Gene 5434 Human
  UniProt ID P19388
• Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in twofold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.
• Synonyms: RNA polymerases I, II, and III subunit ABC1, RPABC1, XAP4

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase