PKC theta (PRKCQ) Rabbit Polyclonal Antibody

CAT#: AP20760PU-N

PKC theta (PRKCQ) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of PKC ? antibody (Cat.-No.: AP20760PU-N) in extracts from A549 cells.

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  Immunohistochemistry (IHC) analyzes of PKC theta antibody (Cat.-No.: AP20760PU-N) in paraffin-embedded human skeletal muscle tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of PKC theta protein.
  (region surrounding asn670)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 80 kDa
• Gene Name: protein kinase C theta
• Database Link:
  Entrez Gene 18761 MouseEntrez Gene 85420 RatEntrez Gene 5588 Human
  UniProt ID Q04759
• Background: Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon , zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilonare independent of Ca++. On the other hand, nPKC and epsilon, as well as all of the cPKC members, possess phorbol esterbinding activities and kinase activities.
• Synonyms: PKC theta, PKC-theta, nPKC-theta, Protein kinase C theta type

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, T cell receptor signaling pathway, Tight junction, Vascular smooth muscle contraction