AKT2 Rabbit Polyclonal Antibody

CAT#: AP20689PU-N

AKT2 rabbit polyclonal antibody, Aff - Purified


USD 443.00

3 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Applications IF, IHC, WB
Recommended Dilution Western blot: 1/500-1/1000.
Immunohistochemistry on paraffin sections: 1/50-1/200.
Immunofluorescence: 1/50-1/200.
Reactivities Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide, corresponding to amino acids 450-500 of Human AKT2.
Specificity AKT2 (T468) pAb detects endogenous levels of total AKT2 protein. This antibody does not cross-react with AKT1 or AKT3.
Formulation Phosphate buffered saline (PBS), pH 7.2
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
Preservative: 0.05% sodium azide
Concentration 1.0 mg/ml
Purification Affinity Chromatography using epitope-specific immunogen
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Predicted Protein Size ~ 60 kDa
Gene Name Homo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2), transcript variant 1
Background The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-I (IGF-I) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-I-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor Wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. Akt3 is phosphorylated on a serine residue in response to insulin, and this activation is inhibited by prior activation of protein kinase C.
Synonyms RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
Protein Pathways Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.