Nephrin (NPHS1) Rabbit Polyclonal Antibody

CAT#: AP20587PU-N

Nephrin (NPHS1) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry (IHC) analyzes of Nephrin antibody (Cat.-No.: AP20587PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Nephrin protein.
  (region surrounding His892)
• Formulation: PBS, pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 135 kDa
• Gene Name: NPHS1 nephrin
• Database Link:
  Entrez Gene 54631 MouseEntrez Gene 64563 RatEntrez Gene 4868 Human
  UniProt ID O60500
• Background: Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with Phorbol-12-myristate- 13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin. Nephrin identified in mouse glomerular extract is a protein with an apparent molecular mass of 185 kDa.
• Synonyms: NPHS1, NPHN

Reference Data

• Protein Families: Druggable Genome, Transmembrane