53BP1 (TP53BP1) Rabbit Polyclonal Antibody

CAT#: AP20288PU-N

53BP1 (TP53BP1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of 53BP1 antibody in extracts from A549 cells.

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  Western blot analysis of 53BP1 antibody at 1/500 dilution: Lane 1: Hela cell lysate. Lane 2: sp2/0 cell lysate. Lane 3: Rat Kidney tissue lysate. Lane 4: PC12 cell lysate.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to the N-terminus of Human 53BP1.
• Specificity: This antibody detects endogenous levels of 53BP1 protein.
• Formulation: PBS, pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~213 kDa
• Gene Name: Homo sapiens tumor protein p53 binding protein 1 (TP53BP1), transcript variant 2
• Database Link:
  Entrez Gene 296099 RatEntrez Gene 7158 Human
  UniProt ID Q12888
• Background: p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.
• Synonyms: p53-binding protein 1, p53BP1, 53BP1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors