DDX3 (DDX3X) Rabbit Polyclonal Antibody

CAT#: AP20221PU-N

DDX3 (DDX3X) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of DDX3 antibody (AP20221PU-N) in extracts from HepG2 cells.

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  Immunohistochemistry analyzes of DDX3 antibody (AP20221PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of DDX3 protein.
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen, purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 75 kDa
• Gene Name: DEAD-box helicase 3, X-linked
• Database Link:
  Entrez Gene 13205 MouseEntrez Gene 317335 RatEntrez Gene 1654 Human
  UniProt ID O00571
• Background: DDX3 contains all of the motifs of the DEAD box family of RNA helicases, including the Asp/Glu/Ala/Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It is also involved in cell growth control and is deregulated in hepatitis virus associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.
• Synonyms: DBX, DDX3, HLP2, Helicase-like protein 2, DEAD box X isoform, DEAD3

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: RIG-I-like receptor signaling pathway