PPP2R2B (110-139) Rabbit Polyclonal Antibody

CAT#: AP18157PU-N

PPP2R2B (110-139) rabbit polyclonal antibody

Product Images

Western blot analysis of PPP2R2B Antibody (Center) in Hela cell line lysates (35ug/lane). PPP2R2B (arrow) was detected using the purified Pab.

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  PPP2R2B Antibody staining of Formalin-fixed and paraffin-embedded human brain tissue using peroxidase-conjugated secondary antibody, followed by DAB staining.

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  Immunofluorescence analysis of PPP2R2B Antibody (Center) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated Goat anti-Rabbit IgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

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  Flow Cytometric analysis of hela cells using PPP2R2B Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Immunohistochemistry: 1/50-1/100.
  Immunofluorescence: 1/10-1/50.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 110~139 amino acids from the Center region of Human PPP2R2B.
• Specificity: This antibody recognizes PPP2R2B.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography followed by peptide Affinity purification.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein phosphatase 2 regulatory subunit Bbeta
• Database Link:
  Entrez Gene 5521 Human
  UniProt ID Q00005
• Background: PPP2R2B belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This protein is a beta isoform of the regulatory subunit B55 subfamily.
• Synonyms: PP2A subunit B B-beta isoform, B55-beta isoform, PR55-beta isoform, R2-beta isoform, protein phosphatase 2A 55 kDa regulatory subunit B beta
• Note: Calculated Molecular Weight: 51710 Da

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction