PTGIS (C-term) Rabbit Polyclonal Antibody

CAT#: AP17980PU-N

PTGIS (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of PTGIS Antibody (C-term) in CEM cell line lysates (35ug/lane). CYP8A1 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human prostate carcinoma reacted withPTGIS Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/1,000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 472-500 amino acids from the C-terminal region of Human CYP8A1.
• Specificity: This antibody recognizes PTGIS.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 57104 kDa
• Gene Name: prostaglandin I2 (prostacyclin) synthase
• Database Link:
  Entrez Gene 5740 Human
  UniProt ID Q16647
• Background: CYP8A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis.
• Synonyms: Prostacyclin Synthase, CYP8, CYP8A1

Reference Data

• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways