VMAT2 (SLC18A2) (C-term) Goat Polyclonal Antibody

CAT#: AP16297PU-N

VMAT2 (SLC18A2) (C-term) goat polyclonal antibody, Aff - Purified

Product Images

AP16297PU-N (1ug/ml) staining of Human Placenta (A), Human Uterus (B), Human Adrenal Gland (C) and Human Pancreas (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  AP16297PU-N (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: IHC, PEP-ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western Blot: 1-3 µg/ml. Approx 55kDa band observed in Human Placenta and Adrenal Gland lysates (calculated MW of 55.7kDa according to NP_003045.2). Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch.
  Immunohistochemistry on Paraffin Sections: 2-5 µg/ml.
  In Paraffin Embedded Human Adrenal Gland shows cytoplasm staining of some epithelial cells in the medula.
  In paraffin embedded Human Adrenal Gland expression of SLC18A2 is detected in chromaffin cells.
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_003045.2.
• Specificity: This antibody recognizes VMAT2.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: solute carrier family 18 member A2
• Database Link:
  Entrez Gene 6571 Human
  UniProt ID Q05940
• Background: The vesicular monoamine transporter is responsible for the vesicular uptake of monoamines, like dopamine, norepinephrine, epinephrine, serotonin and histamine. The antiserum recognizes monoaminergic neurons of the CNS, the ECL-cells of the stomach, as well as enteric nerve fibers.
• Synonyms: SVMT, VAT2