CCK4 (PTK7) (N-term) Rabbit Polyclonal Antibody

CAT#: AP14595PU-N

CCK4 (PTK7) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of CCK4 (arrow) using rabbit polyclonal CCK4 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/50 - 1/200.
  Immunohistochemistry: 1/50 - 1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CCK4.
• Specificity: This antibody reacts to CCK4 (PTK7).
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein tyrosine kinase 7 (inactive)
• Database Link:
  Entrez Gene 5754 Human
  UniProt ID Q13308
• Background: CCK4 may function as a cell adhesion molecule. Although it belongs to the insuline receptor subfamily of the Tyr protein kinases, it likely lacks the catalytic activity of a tyrosine kinase. It may be connected to the pathophysiology of colon carcinomas and/or may represent a tumor progression marker. This Type I membrane protein is highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes, but weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle, and not in colon. It is also expressed in erythroleukemia cells.
• Synonyms: Tyrosine-protein kinase-like 7, Colon carcinoma kinase 4, CCK-4