JLP (SPAG9) (N-term) Rabbit Polyclonal Antibody

CAT#: AP14021PU-N

JLP (SPAG9) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of SPAG9 (arrow) using rabbit polyclonal SPAG9 (N-term) Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 (N-term) gene (Lane 2) (Origene Technologies).

• 

  Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/50-1/100.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 98~128 amino acids from the N-terminal region of Human SPAG9
• Specificity: This antibody detects Human SPAG9 at N-term.
  Other soecies not tested
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: sperm associated antigen 9
• Database Link:
  Entrez Gene 9043 Human
  UniProt ID O60271
• Background: SPAG9, which is abundantly expressed in testicular haploid germ cells, is recognized by sperm-agglutinating antibodies and implicated in infertility.
• Synonyms: Sperm-associated antigen 9, Sperm-specific protein, Sperm surface protein, JNK-interacting protein 4, JIP-4, HSS, KIAA0516, MAPK8IP4, SYD1, HLC6, HLC-6, PHET, CT89, Sunday driver 1
• Note: Molecular weight: 146205 Da