AMPK alpha 2 (PRKAA2) (C-term) Rabbit Polyclonal Antibody

CAT#: AP13880PU-N

AMPK alpha 2 (PRKAA2) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-AMPK alpha2 C-term Pab in mouse heart tissue lysate. AMPK alpha2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:100 - 1:500.
  Flow cytometry.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AMPK.
• Specificity: This antibody detects AMPK alpha2 at C-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein kinase AMP-activated catalytic subunit alpha 2
• Database Link:
  Entrez Gene 5563 Human
  UniProt ID P54646
• Background: AMPK2 alpha2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
• Synonyms: AMPK2, AMPK alpha-2 chain
• Note: Molecular weight: 62319 Da.