CDC25B pSer187 Rabbit Polyclonal Antibody
CDC25B pSer187 rabbit polyclonal antibody, Aff - Purified
|Recommended Dilution||ELISA: 1/1,000.
Western Blot: 1/100-1/500.
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S187 of human Cdc25B.|
|Specificity||This antibody detects CDC25B pSer187.|
|Formulation||PBS with 0.09% (W/V) Sodium Azide as preservative.
State: Aff - Purified
State: Liquid purified Ig fraction.
|Purification||Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Gene Name||cell division cycle 25B|
|Background||CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.|
|Note||Molecular weight: 64987 Da|
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