CD73 (NT5E) (N-term) Rabbit Polyclonal Antibody

CAT#: AP11872PU-N

CD73 (NT5E) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-NT5E antibody in cell line lysate (35 ug/lane). NT5E (arrow) was detected using the purified Pab.

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  Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human kidney tissue stained with CD73 (NT5E) Antibody (N-term) ( followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CD73 (NT5E) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human NT5E
• Specificity: This antibody detects CD73 (NT5E) at N-term.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: 5'-nucleotidase ecto
• Database Link:
  Entrez Gene 4907 Human
  UniProt ID P21589
• Background: Ecto-5-prime-nucleotidase catalyzes conversion at neutral pH of purine 5-prime mononucleotides to nucleosides. The enzyme consists of a dimer of 2 identical 70 kD subunits tethered by a glycosyl phosphatidyl inositol moeity to the exterior plasma membrane surface. The enzyme, a marker of lymphocyte differentiation is associated in deficiency with a variety of immunodeficiency diseases. The preferred substrate is AMP. The NT5 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations.
• Synonyms: NT5, NTE, NT5E
• Note: Calculated Molecular Weight: 63368 Da