dcr-1 (Center) Rabbit Polyclonal Antibody

CAT#: AP11794PU-N

dcr-1 (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Dcr-1 antibody (Center) in CEM cell line lysates (35ug/lane). Dcr-1 (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/1000.
• Reactivities: C. elegans
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1068~1097 amino acids from the central region of Human Dcr-1
• Specificity: This antibody detects Dcr-1 at Center.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  UniProt ID P34529
• Background: The Dcr-1 gene encodes a bidentate ribonuclease that is homologous to E. coli RNAse III. Dcr-1 is required both for RNA interference and for synthesis of small developmental RNAs. Dcr-1 interacts in vivo with RDE-4, a double-stranded RNA (dsRNA) binding protein required for RNAi that interacts with trigger dsRNAs and may function to deliver dsRNAs to Dcr-1 for endonucleolytic processing.
• Note: Molecular weight: 210924 Da