EIF4B Rabbit Polyclonal Antibody

CAT#: AP11771PU-N

EIF4B rabbit polyclonal antibody, Purified

Product Images

Western blot analysis using EIF4B Antibody (Center) in HepG2 cell line lysates (35 ug/lane). EIF4B (arrow) was detected using the purified Pab at 1/60 dilution.

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  Western blot analysis using EIF4B Antibody (Center) in 293 cell line lysates transiently transfected with the EIF4B gene (2 ug/lane). EIF4B (arrow) was detected using the purified Pab at 1/60 dilution.

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  EIF4B antibody staining of Formalin-Fixed, Paraffin-Embedded Human breast carcinoma. This antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:50 - 1:100
  Immunohistochemistry 1:50 - 1:100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human EIF4B Antibody.
• Specificity: This antibody detects EIF4B at center.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: eukaryotic translation initiation factor 4B
• Database Link:
  Entrez Gene 1975 Human
  UniProt ID P23588
• Background: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
• Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B
• Note: Molecular weight: 69151 Da