CD45 (PTPRC) (C-term) Rabbit Polyclonal Antibody

CAT#: AP11621PU-N

CD45 (PTPRC) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot using CD45 Antibody to detect CD45 in jurkat cell lysate.

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  Formalin-Fixed Paraffin-Embedded Human tonsil reacted with CD45 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for Immunohistochemistry.

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  Flow Cytometric analysis of jurkat cells using CD45 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1252~1282 amino acids from the C-terminal region of Human CD45.
• Specificity: This antibody detects CD45 at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein tyrosine phosphatase, receptor type C
• Database Link:
  Entrez Gene 5788 Human
  UniProt ID P08575
• Background: CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. The CD45 gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200
• Note: Calculated Molecular Weight: 147253 Da