BCL2 (BH3 Domain) Rabbit Polyclonal Antibody

CAT#: AP11295PU-N

BCL2 (BH3 Domain) rabbit polyclonal antibody, Purified

Product Images

Left panel: Analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using antibody for immunoprecipitation (IP) and a different Bcl-2 antibody for western blot. Right panel : Same analysis using in Flow Cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/100-1/500.
  Immunohistochemistry: 1/50-1/100.
  Immunoprecipitation: 1/100-1/500
  Flow Cytometry: 1/100-1/500.
  Immunofluorescence.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the BH3 domain of human Bcl2.
• Specificity: This antibody is specific to Bcl-2 (BH3 Domain).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: B-cell CLL/lymphoma 2
• Database Link:
  Entrez Gene 596 Human
  UniProt ID P10415
• Background: BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.
• Synonyms: BCL2, Bcl-2 alpha
• Note: Calculated MW: 26266 Da