TrkA (NTRK1) Rabbit Polyclonal Antibody

CAT#: AP09509PU-N

TrkA (NTRK1) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunofluorescence staining of methanol-fixed HeLa cells using TrkA Antibody

Specifications

Product Data

• Applications: IF
• Recommended Dilution: Immunofluorescence: 1/100-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-Peptide sequence around aa.789~793 (P-V-Y-L-D) derived from Human TrkA.
• Specificity: This antibody detects endogenous levels of total TrkA protein.
• Formulation: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), transcript variant 3
• Database Link:
  Entrez Gene 4914 Human
  UniProt ID P04629
• Background: The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.
• Synonyms: NTRK1, TRK, Trk-A

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Apoptosis, Endocytosis, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Thyroid cancer