Cyclin B1 (CCNB1) pSer147 Rabbit Polyclonal Antibody

CAT#: AP09487PU-N

Cyclin B1 (CCNB1) pSer147 rabbit polyclonal antibody, Aff - Purified

Size: 50 ul 100 ul


USD 403.00

2 Weeks*

Size
    • 100 ul

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Specifications

Product Data
Applications IF, IHC, WB
Recommended Dilution Immunofluorescence: 1/100 - 1/200.
Immunohistochemistry on Paraffin Sections: 1/50 - 1/100.
Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-Sp-D-V).
Specificity This Antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Serine 147.
Formulation PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State: Aff - Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Immunoaffinity Chromatography
Conjugation Unconjugated
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Gene Name Homo sapiens cyclin B1 (CCNB1)
Background In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Synonyms CCNB1, CCNB, Cyclin-B1
Reference Data
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.