Phospholipase C gamma 1 (PLCG1) pTyr771 Rabbit Polyclonal Antibody

CAT#: AP09485PU-N

Phospholipase C gamma 1 (PLCG1) pTyr771 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-gamma1 (pTyr771) antibody

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  Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using PLCG1 pTyr771 Antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Immunofluorescence: 1/100 - 1/200.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human PLCG1 around the phosphorylation site of Tyrosine 771 (P-D-Yp-G-A).
• Specificity: Antibody AP09485PU detects endogenous levels of PLC-gamma-1 only when phosphorylated at Tyrosine 771.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: phospholipase C gamma 1
• Database Link:
  Entrez Gene 18803 MouseEntrez Gene 25738 RatEntrez Gene 5335 Human
  UniProt ID P19174
• Background: Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.
• Synonyms: PLC1, Phospholipase C-gamma-1, PLC-gamma-1, PLC gamma1 Phospholipase C-II, PLC-II, PLC-148

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, T cell receptor signaling pathway, VEGF signaling pathway, Vibrio cholerae infection