EIF4B pSer422 Rabbit Polyclonal Antibody

CAT#: AP09472PU-N

EIF4B pSer422 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from Hela cells untreated or treated with EGF using eIF4B pSer422 Antibody.

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B pSer422 antibody (Left) and the same antibody preincubated with blocking peptide (Right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from Human eIF4B around the phosphorylation site of Serine 422 (T-G-Sp-E-S).
• Specificity: This antibody detects endogenous levels of eIF4B only when phosphorylated at Serine 422.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: eukaryotic translation initiation factor 4B
• Database Link:
  Entrez Gene 75705 MouseEntrez Gene 1975 Human
  UniProt ID P23588
• Background: eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
• Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B
• Note: Molecular Weight: 80 kDa

Reference Data

• Protein Pathways: mTOR signaling pathway