FITC Goat Polyclonal Antibody

SKU AP09203PU-N

FITC goat polyclonal antibody, Aff - Purified

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.; Recommended Dilutions:; ELISA: 1/5,000-1/30,000.; Western Blot: 1/500-1/3,000.; Immunohistochemistry: 1/250-1/1,500.
• Antibody Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fluorescein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative.; State: Aff - Purified; State: Liquid (sterile filtered) purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Background: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
• Synonyms: Fluorescein Isothiocyanate

Reference Data