Biotin Goat Polyclonal Antibody

CAT#: AP09114FC-N

Biotin goat polyclonal antibody, DTAF

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Immunofluorescence.
  Flow Cytometry.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Biotin conjugated Keyhole Limpet Hemocyanin (KLH)
• Specificity: This antibody reacts to Fluorescein conjugated IgG fraction of Biotin.
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: DTAF
  State: Lyophilized IgG fraction
  Label: Diaminotriazynlaminofluorescein
  Absorption emission: 495 nm / 528 nm
  Molar radio: 1.7 moles DTAF per mole of Goat IgG
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Conjugation: DTAF
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
• Synonyms: Vitamin B7, Vitamin H