Axin 1 (AXIN1) (850-862) Goat Polyclonal Antibody

CAT#: AP08956PU-N

Axin 1 (AXIN1) (850-862) goat polyclonal antibody, Aff - Purified

Product Images

Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: ELISA: 1/128000.
  Immunohistochemistry on Paraffin Sections: 3.75 µg/ml.
• Reactivities: Human, Mouse, Rat, Canine, Bovine, Bat, Equine, Hamster, Monkey, Zebrafish
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from C-terminus of Human AXIN1
• Specificity: This antibody recognizes Axin 1 (AXIN1) at aa 850-862. It is expected to recognise both reported isoforms.
• Formulation: Tris-Saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: axin 1
• Database Link:
  Entrez Gene 8312 Human
  UniProt ID O15169
• Background: Axin-1 (Axis inhibition protein 1) and Axin-2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin-1 interacts with APC, GSK3-beta, Dvl and beta-Catenin and promotes the GSK3-beta-mediated phosphorylation and subsequent degradation of beta-Catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute Axin1 in mice (6). Axin-2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).
• Synonyms: AXIN1, AXIN