Iba1 (AIF1) (149-161) Goat Polyclonal Antibody

CAT#: AP08912PU-N

Iba1 (AIF1) (149-161) goat polyclonal antibody, Aff - Purified

Product Images

AIF1 Antibody (0.5 ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Formalin-Fixed, Paraffin-Embedded Human Lung staining using AIF1 Antibody at 5 ug/ml after heat-induced antigen retrieval.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/128000.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 0.3 - 1 µg/ml.
• Reactivities: Hamster, Human, Monkey, Mouse, Porcine, Rat, Bovine, Equine, Goat, Rabbit
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from C-terminus of Human AIF1 (NP_116573.1; NP_001614.3).
  Epitope: aa 149-161
  
• Specificity: This antibody recognizes Allograft Inflammatory Factor-1 (AIF1) at aa 149-161.
  The antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
• Formulation: Tris saline buffer, pH ~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: allograft inflammatory factor 1
• Database Link:
  Entrez Gene 199 Human
  UniProt ID P55008
• Background: Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.
• Synonyms: AIF-1, Protein G1, IBA1, Allograft inflammatory factor 1