CAMK2A pThr286 Rabbit Polyclonal Antibody

CAT#: AP08911PU-N

CAMK2A pThr286 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot of rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.

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  Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE):

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 1/200.
  Western Blot: 1/1000.
• Reactivities: Human, Mouse, Rat, Xenopus
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in Rat brain CaM Kinase II.
• Specificity: This antibody recognizes Calcium/Calmodulin-dependent Protein Kinase Type II (CAMK2).
  Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment.
• Formulation: 10mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol
  State: Aff - Purified
  State: Liquid Purified Ig fraction
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: calcium/calmodulin dependent protein kinase II alpha
• Database Link:
  Entrez Gene 12322 MouseEntrez Gene 25400 RatEntrez Gene 815 Human
  UniProt ID Q9UQM7
• Background: CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits.
  CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.
• Synonyms: CAMK2A, CAMK2B, CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-II subunit alpha, CaM-kinase II beta chain, CaM kinase II subunit beta, CaMK-II subunit beta

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway