Ogg1 Goat Polyclonal Antibody

CAT#: AP08789PU-N

Ogg1 goat polyclonal antibody, Aff - Purified

Product Images

Figure 2. Antibody ( at 0.1 ug/ml) staining of Rat Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by Chemiluminescence.

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  Figure 1. Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded Kidney tissue using at 2.5 ug/ml.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/128000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/128000, 0.1 - 0.3 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide (C-HPKTSQAKGPSPLANK) from an Internal region of Mouse OGG1.
• Specificity: Recognizes 8-oxoguanine DNA Glycosylase Homolog 1 (OGG1).
• Formulation: Tris Saline Buffer, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: 8-oxoguanine DNA glycosylase
• Database Link:
  Entrez Gene 81528 Rat
  UniProt ID O70249
• Background: The Ogg1 gene encodes a DNA repair enzyme (N glycosylase/DNA lyase) that incises DNA at 8 oxoG residues. N glycosylase/DNA lyase excises 7,8 dihydro 8 oxoguanine and 2,6 diamino 4 hydroxy 5 N methylformamidopyrimidine (FAPY) from damaged DNA. N glycosylase/DNA lyase has a beta lyase activity that nicks DNA 3' to the lesion.
  Alternative splicing of the C terminal region of the Ogg1 gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N terminal region in common. Many alternative splice variants for this gene have been described, but the full length nature for every variant has not been determined. The N terminus of this gene contains a mitochondrial targetting signal, essential for mitochondrial localization.
• Synonyms: MMH, MUTM, OGH1, N-glycosylase/DNA lyase